Self-Interaction Chromatography (SIC) on-Column Binding Optimization for Pre-Packed Columns

Friday, November 7, 2014
Plaza Foyer (Sheraton)
Sara Liaghati-Mobarhan , University of Alabama at Birmingham
On-column protein binding can be used to provide a less labor and time intensive process for the preparation and completion of miniaturized SIC through the comparison of on-column and batch-bound protein columns. A protocol can be developed that ensures analogous or improved column homogeneity and calculated B-values.