Introducing Stabilization Mutations K82D and N108K in Single-Chain Human Hemoglobin α1-β1 Dimer

My research focuses on developing single-chain hemoglobin as a potential blood substitute for use in critical care. Three amino acids were substituted via single nucleotide polymorphisms in the permuted α1-β1 dimer globin with the intent to stabilize the T-state and create a more biologically compatible hemoglobin mutant.