Designing Tricistronic and Bicistronic Lenti-Viral Vectors Using Scar-Less DNA Assembly Methods and Web-Based Software to Study GRK4

Friday, November 7, 2014
Plaza Foyer (Sheraton)
Christophe Langouet-Astrie , James Madison University
John Gildea , University of Virginia
The purpose is to construct tricistronic lenti-viral vectors containing G protein-coupled receptor kinase 4 in order to help develop an assay for detecting salt sensitivity. Vectors are constructed using novel gene cloning techniques to optimize cost and time efficiency and utilizing a web-based software to reduce construction difficulty.