Designing Tricistronic and Bicistronic Lenti-Viral Vectors Using Scar-Less DNA Assembly Methods and Web-Based Software to Study GRK4

The purpose is to construct tricistronic lenti-viral vectors containing G protein-coupled receptor kinase 4 in order to help develop an assay for detecting salt sensitivity. Vectors are constructed using novel gene cloning techniques to optimize cost and time efficiency and utilizing a web-based software to reduce construction difficulty.