Synthesis of a Novel Universal Fluorescent DNA Nucleoside

The goal of this project is to synthesize a universal fluorescent DNA base. Our work has focused on the synthesis of dimethylaminonaphthylimide (DANI) and 1-α-Chloro-1,2-dideoxy-3,5-di-O-toluoyl ribose. DANI will be attached to the sugar, then used for kinetic analysis of Bovine Leukemia Virus. The synthesis of the sugar-base complex will be reported.